Please use this identifier to cite or link to this item: https://doi.org/10.1002/bit.24343
Title: Branched polyethylene glycol for protein precipitation
Authors: Sim, S.-L.
He, T.
Tscheliessnig, A.
Mueller, M.
Tan, R.B. 
Jungbauer, A.
Keywords: Branched
Hydrodynamic radius
Polyethylene glycol
Precipitation
Protein
Viscosity
Issue Date: Mar-2012
Citation: Sim, S.-L., He, T., Tscheliessnig, A., Mueller, M., Tan, R.B., Jungbauer, A. (2012-03). Branched polyethylene glycol for protein precipitation. Biotechnology and Bioengineering 109 (3) : 736-746. ScholarBank@NUS Repository. https://doi.org/10.1002/bit.24343
Abstract: The use of linear PEGs for protein precipitation raises the issues of high viscosity and limited selectivity. This paper explores PEG branching as a way to alleviate the first problem, by using 3-arm star as the model branched structure. 3-arm star PEGs of 4,000 to 9,000Da were synthesized and characterized. The effects of PEG branching were then elucidated by comparing the branched PEG precipitants to linear versions of equivalent molecular weights, in terms of IgG recovery from CHO cell culture supernatant, precipitation selectivity, solubility of different purified proteins, and precipitation kinetics. Two distinct effects were observed: PEG branching reduced dynamic viscosity; secondly, the branched PEGs precipitated less proteins and did so more slowly. Precipitation selectivity was largely unaffected. When the branched PEGs were used at concentrations higher than their linear counterparts to give similar precipitation yields, the dynamic viscosity of the branched PEGs were noticeably lower. Interestingly, the precipitation outcome was found to be a strong function of PEG hydrodynamic radius, regardless of PEG shape and molecular weight. These observations are consistent with steric mechanisms such as volume exclusion and attractive depletion. © 2011 Wiley Periodicals, Inc.
Source Title: Biotechnology and Bioengineering
URI: http://scholarbank.nus.edu.sg/handle/10635/88593
ISSN: 00063592
DOI: 10.1002/bit.24343
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

15
checked on Jul 9, 2018

WEB OF SCIENCETM
Citations

12
checked on Jul 9, 2018

Page view(s)

22
checked on May 18, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.