Please use this identifier to cite or link to this item: https://doi.org/10.1080/08927010902875121
Title: Bifunctional oligo(ethylene glycol) decorated surfaces which permit covalent protein immobilization and resist protein adsorption
Authors: Bi, X. 
Xu, H. 
Lai, S.L.
Yang, K.-L. 
Keywords: Biofouling
Nonspecific adsorption
Oligo(ethylene glycol)
Protein immobilization
Issue Date: Jul-2009
Source: Bi, X., Xu, H., Lai, S.L., Yang, K.-L. (2009-07). Bifunctional oligo(ethylene glycol) decorated surfaces which permit covalent protein immobilization and resist protein adsorption. Biofouling 25 (5) : 435-444. ScholarBank@NUS Repository. https://doi.org/10.1080/08927010902875121
Abstract: In this article, surface coatings derived from homo-bifunctional tri(ethylene glycol) (EG3) and hexa(ethylene glycol) (EG6) molecules which have two terminal aldehyde groups are reported. These homo-bifunctional molecules can be used to functionalize amine-terminated surfaces through crosslinking one aldehyde group to surface amine groups, while leaving the other aldehyde group available for covalent immobilization of proteins. Best of all, after reducing remaining aldehyde groups on the surface with a reducing agent, sodium borohydride, the surface becomes oligo(ethylene glycol) (OEG)-terminated. The OEG-terminated surface can resist nonspecific protein adsorption, a feature that is often required for many biosensors and biomedical devices. Although some mixed self-assembled monolayers formed from two different organothiols also permit covalent protein immobilization and resist nonspecific protein adsorption, the procedure reported herein requires only one type of homo-bifunctional molecule and can be applied to both silicon and gold surfaces. © 2009 Taylor & Francis.
Source Title: Biofouling
URI: http://scholarbank.nus.edu.sg/handle/10635/88576
ISSN: 08927014
DOI: 10.1080/08927010902875121
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