Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jcis.2007.08.012
Title: Adsorption, desorption, and conformational changes of lysozyme from thermosensitive nanomagnetic particles
Authors: Shamim, N.
Liang, H. 
Hidajat, K. 
Uddin, M.S. 
Keywords: Conformational changes
Isoelectric point
Lower critical solution temperature (LCST)
Lysozyme
N-isopropylacrylamide
Issue Date: 1-Apr-2008
Source: Shamim, N., Liang, H., Hidajat, K., Uddin, M.S. (2008-04-01). Adsorption, desorption, and conformational changes of lysozyme from thermosensitive nanomagnetic particles. Journal of Colloid and Interface Science 320 (1) : 15-21. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jcis.2007.08.012
Abstract: Adsorption of globular protein, lysozyme, on thermosensitive poly(N-isopropylacrylamide) coated nanomagnetic particles was studied at different temperatures and pHs. It was observed that a maximum amount of lysozyme was adsorbed at a temperature above the lower critical solution temperature (LCST) (32 °C ) of the polymer and at the isoelectric point (p I = 11) of lysozyme. Desorption was carried out using either NaH2PO4 (pH 4) or NaSCN (pH 6) as the desorbing agents. Conformational changes in lysozyme on desorption from nanomagnetic particles was studied by circular dichroism and intrinsic fluorescence spectroscopy. Lysozyme desorbed by NaH2PO4 showed very little conformational changes while lysozyme desorbed by NaSCN showed significant conformational changes, and 87% enzymatic activity was retained in the desorbed enzyme for desorption by NaH2PO4. © 2007 Elsevier Inc. All rights reserved.
Source Title: Journal of Colloid and Interface Science
URI: http://scholarbank.nus.edu.sg/handle/10635/88508
ISSN: 00219797
DOI: 10.1016/j.jcis.2007.08.012
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

48
checked on Mar 6, 2018

WEB OF SCIENCETM
Citations

50
checked on Mar 6, 2018

Page view(s)

31
checked on Apr 20, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.