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|Title:||Adsorption and desorption of lysozyme on nano-sized magnetic particles and its conformational changes|
Nano-sized magnetic particles
|Citation:||Peng, Z.G., Hidajat, K., Uddin, M.S. (2004-06-01). Adsorption and desorption of lysozyme on nano-sized magnetic particles and its conformational changes. Colloids and Surfaces B: Biointerfaces 35 (3-4) : 169-174. ScholarBank@NUS Repository. https://doi.org/10.1016/j.colsurfb.2004.03.010|
|Abstract:||Adsorption and desorption of lysozyme on nano-sized magnetic particles and its conformational change were studied in this work. Adsorption of lysozyme on nano-sized magnetic particles (Fe3O4) was carried out at different pH. Maximum adsorption of lysozyme (4.65 mg/m2) occurred at its isoelectric point (pI=11.1). Differential scanning calorimetry (DSC) results show that the lysozyme adsorbed on magnetic particles did not show any thermal transition over the range 20-100°C. High desorption of lysozyme from magnetic particles was achieved using NaH2PO4 (pH 4.0) (90%) and NaSCN (pH 6.0) (97%) as desorbents. The conformational change of the lysozyme desorbed by NaH2PO4 was small, while the lysozyme desorbed by NaSCN underwent a significant conformational change as measured by the intrinsic fluorescence. Eighty-eight and 82% activity was retained in the desorbed enzyme for desorption by NaH2PO4 and NaSCN, respectively. © 2004 Elsevier B.V. All rights reserved.|
|Source Title:||Colloids and Surfaces B: Biointerfaces|
|Appears in Collections:||Staff Publications|
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