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|Title:||A liquid crystal-based sensor for real-time and label-free identification of phospholipase-like toxins and their inhibitors|
|Authors:||Hartono, D. |
|Citation:||Hartono, D., Lai, S.L., Yang, K.-L., Yung, L.-Y.L. (2009-03-15). A liquid crystal-based sensor for real-time and label-free identification of phospholipase-like toxins and their inhibitors. Biosensors and Bioelectronics 24 (7) : 2289-2293. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2008.11.021|
|Abstract:||We report a liquid crystal (LC)-based sensor for real-time and label-free identification of phospholipase-like toxins. Beta-bungarotoxin exhibits Ca2+-dependent phospholipase A2 activity whereas alpha-bungarotoxin and myotoxin II do not exhibit any phospholipase activity. The sensor can selectively identify beta-bungarotoxin, when it hydrolyzes a phospholipid monolayer self-assembled at aqueous-LC interface, through orientational responses of LCs. As a result, optical signals that reflect the spatial and temporal distribution of phospholipids during the hydrolysis can therefore be generated in a real-time manner. The sensor is very sensitive and requires less than 5 pg of beta-bungarotoxin for the detection. When phospholipase A2 inhibitors are introduced together with beta-bungarotoxin, no orientational response of LCs can be observed. In addition, the regeneration of the sensor can be done without affecting the sensing performance. This work demonstrates a simple and cost-effective LC-based sensor for identifying phospholipase-like toxins and for screening compound libraries to find potential toxin inhibitors. © 2008 Elsevier B.V. All rights reserved.|
|Source Title:||Biosensors and Bioelectronics|
|Appears in Collections:||Staff Publications|
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