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|Title:||Use of IR-to-visible upconversion fluorescent nanoparticles for tracking of siRNA delivery|
|Citation:||Jiang, S.,Zhang, Y. (2008). Use of IR-to-visible upconversion fluorescent nanoparticles for tracking of siRNA delivery. Proceedings of the 6th IASTED International Conference on Biomedical Engineering, BioMED 2008 : 368-371. ScholarBank@NUS Repository.|
|Abstract:||IR-to-visible upcoversion fluorescent nanoparticles (UCNs) were developed to track in vitro delivery of small interference RNA (siRNA) and meanwhile efficiently transfect siRNA to the cells. NaYF4 upconversion nanoparticles co-doped with Yb/Tm (NaYF4:Yb,Tm) were conjugated with GL3 siRNA through electrostatic attraction at the weight ratio of 8:3. Then, siRNA- conjugated UCNs were incubated with the cultured human breast cancer MCF-7 cells. After 24h-incubation the successful cellular uptake of siRNA was visualized through the emitted blue fluorescence of siRNA- conjugated UCNs under confocal laser scanning microscopy. After 48h-incubation the efficiency of delivered GL3 siRNA in the inhibition of luciferase gene was ~60% that was determined by luciferase assay. This article provided initial insights into the effectiveness and suitability of NaYF4:Yb,Tm upconversion nanoparticles as an easily detectable and self-tracking delivery vector of siRNA.|
|Source Title:||Proceedings of the 6th IASTED International Conference on Biomedical Engineering, BioMED 2008|
|Appears in Collections:||Staff Publications|
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