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|Title:||Simultaneous stimulated Raman scattering and higher harmonic generation imaging for liver disease diagnosis without labeling|
multimodal nonlinear optical microscopy
second harmonic generation
stimulated Raman scattering
third harmonic generation
|Source:||Lin, J., Wang, Z., Zheng, W., Huang, Z. (2014). Simultaneous stimulated Raman scattering and higher harmonic generation imaging for liver disease diagnosis without labeling. Progress in Biomedical Optics and Imaging - Proceedings of SPIE 8948 : -. ScholarBank@NUS Repository. https://doi.org/10.1117/12.2039456|
|Abstract:||Nonlinear optical microscopy (e.g., higher harmonic (second-/third- harmonic) generation (HHG), simulated Raman scattering (SRS)) has high diagnostic sensitivity and chemical specificity, making it a promising tool for label-free tissue and cell imaging. In this work, we report a development of a simultaneous SRS and HHG imaging technique for characterization of liver disease in a bile-duct-ligation rat-modal. HHG visualizes collagens formation and reveals the cell morphologic changes associated with liver fibrosis; whereas SRS identifies the distributions of hepatic fat cells formed in steatosis liver tissue. This work shows that the co-registration of SRS and HHG images can be an effective means for label-free diagnosis and characterization of liver steatosis/fibrosis at the cellular and molecular levels. © 2014 SPIE.|
|Source Title:||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|Appears in Collections:||Staff Publications|
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