Please use this identifier to cite or link to this item:
Title: Multimodal nonlinear optical microscopy for label-free imaging of liver tissue
Authors: Lin, J.
Zheng, W. 
Wang, Z.
Huang, Z. 
Issue Date: 2013
Source: Lin, J.,Zheng, W.,Wang, Z.,Huang, Z. (2013). Multimodal nonlinear optical microscopy for label-free imaging of liver tissue. Optics InfoBase Conference Papers. ScholarBank@NUS Repository.
Abstract: Liver fibrosis and steatosis represent the complex conditions and major symptoms for many liver diseases. In this study, we applied four modalities of nonlinear optical microscopy imaging on the bile-duct-ligation (BDL) induced fibrosis model of rat livers. Specifically, second harmonic generation (SHG) imaging quantifies the growing of the collagens, the two-photon excited fluorescence (TPEF) imaging reveals the morphology of hepatic cells, coherent anti-Stokes Raman scattering (CARS) imaging maps the distributions of fats or lipids quantitatively across the tissue, and third harmonic generation (THG) imaging is used to visualize the microstructures and morphology changes of the tissue. Our results demonstrate that both liver steatosis and fibrosis occur in the BDL liver tissue; while they develop at different rates. A certain correlationship between the two diseases in the same model can also be revealed. This study may provide new insights into the understanding of the mechanisms of liver disease (steatosis/fibrosis) transformations at the cellular and molecular levels. © The Japan Society of Applied Physics 2013.
Source Title: Optics InfoBase Conference Papers
ISBN: 9784863483699
ISSN: 21622701
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

checked on Feb 23, 2018

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.