Please use this identifier to cite or link to this item: https://doi.org/10.1117/12.2039683
Title: Integrated coherent Raman scattering and multiphoton microscopy for label-free imaging of the dentin in the tooth
Authors: Wang, Z.
Zheng, W. 
Lin, J.
Hsu, C.-Y.
Huang, Z. 
Keywords: dentin
multimodal nonlinear optical microscopy
second harmonic generation
stimulated Raman scattering
third harmonic generation
tooth structure
Issue Date: 2014
Citation: Wang, Z., Zheng, W., Lin, J., Hsu, C.-Y., Huang, Z. (2014). Integrated coherent Raman scattering and multiphoton microscopy for label-free imaging of the dentin in the tooth. Progress in Biomedical Optics and Imaging - Proceedings of SPIE 8948 : -. ScholarBank@NUS Repository. https://doi.org/10.1117/12.2039683
Abstract: We report the implementation of a unique multimodal nonlinear optical microscopy (i.e., coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), third harmonic generation (THG) and two photon excitation fluorescence (TPEF)) platform for label-free imaging of dentin. A picosecond tunable laser together with an OPO is used as the excitation source for simultaneously multimodal imaging. CARS shows similar information as TPEF in dentin, but it has a higher sectioning performance than TPEF and thus it is a good alternative for TPEF. Microtubule structure is revealed nearby dentin enamel junction (DEJ) from the multimodal images. This work demonstrates that combining different nonlinear optical imaging modalities can provide new insights into the understanding of morphological structures and biochemical/biomolecular distributions of the dentine without the need of labeling. © 2014 SPIE.
Source Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
URI: http://scholarbank.nus.edu.sg/handle/10635/88267
ISBN: 9780819498618
ISSN: 16057422
DOI: 10.1117/12.2039683
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