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|Title:||Bead-based DNA Microarray Fabricated on Porous Polymer Films|
|Authors:||Cheng, J.T. |
|Source:||Cheng, J.T.,Li, J.,Chen, N.G.,Gopalakrishnakone, P.,Zhang, Y. (2009). Bead-based DNA Microarray Fabricated on Porous Polymer Films. IFMBE Proceedings 23 : 842-845. ScholarBank@NUS Repository. https://doi.org/10.1007/978-3-540-92841-6_207|
|Abstract:||By attaching different biomolecule probes to surface-modified microspheres, a large number of complementary targets labeled with fluorophores can be interrogated simultaneously using the bead-based microarray format. In this work, a new bead-based DNA microarray was fabricated on a porous polymer film with well-ordered array of pores. The well-ordered porous polymer film was prepared using the non-lithographic breath figure method. Different biotinylated DNA probes were conjugated to avidin-modified polystyrene (PS) microspheres. The DNA microarray was fabricated by randomly dispersing DNA probe-conjugated PS microspheres into the pores on the polymer film. Multiplexing detection of DNA was performed using target DNAs labeled with ATTO dyes using a fluorescence microscope imaging system. The Bead-based DNA microarray was constructed for detection of four serotypes of Dengue virus. This technique was demonstrated to be a simple and cost-effective method for rapid detection of DNA targets. © International Federation of Medical and Biological Engineering 2009.|
|Source Title:||IFMBE Proceedings|
|Appears in Collections:||Staff Publications|
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