Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/88187
DC Field | Value | |
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dc.title | Tissue engineering of a periodontal ligament-alveolar bone graft construct | |
dc.contributor.author | Chou, A.M. | |
dc.contributor.author | Sae-Lim, V. | |
dc.contributor.author | Hutmacher, D.W. | |
dc.contributor.author | Lim, T.M. | |
dc.date.accessioned | 2014-10-08T09:48:32Z | |
dc.date.available | 2014-10-08T09:48:32Z | |
dc.date.issued | 2006-07 | |
dc.identifier.citation | Chou, A.M.,Sae-Lim, V.,Hutmacher, D.W.,Lim, T.M. (2006-07). Tissue engineering of a periodontal ligament-alveolar bone graft construct. International Journal of Oral and Maxillofacial Implants 21 (4) : 526-534. ScholarBank@NUS Repository. | |
dc.identifier.issn | 08822786 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/88187 | |
dc.description.abstract | Purpose: This paper reports on a 2-phase study of a novel membrane-scaffold graft construct, its ability to support periodontal ligament fibroblast (PDLF) and alveolar osteoblast (AO) growth in vitro, and its use for tissue engineering a PDL-AO interface in vivo. Materials and Methods: Human PDLFs were seeded onto perforated poly(ε-caprolactone) membranes (n = 30) at 78,000 cells/cm 2; human AOs were seeded on poly(ε-caprolactone) scaffolds (n = 30) with fibrin glue at 625,000 cells/cm3. Cell attachment, morphology, viability, and metabolic activity were monitored for 3 weeks in vitro. Subsequently, cell-seeded membrane-scaffold constructs (experimental group, n = 9) and nonseeded constructs (control group, n = 4) assembled with fibrin glue were implanted subcutaneously into 7 athymic mice for 4 weeks. Results: PDLFs formed confluent layers on membranes, whereas AOs produced mineralized matrices within scaffolds upon osteoinduction in vitro. Well-vascularized tissue formation was observed after implantation. Integration at the membrane-scaffold interface was enhanced in the experimental group. Type I collagen, type III collagen, fibronectin, and vitronectin were found adjacent to membranes and within constructs. Bone sialoprotein expression and bone formation were undetectable. Discussion: Membrane perforation and scaffold porosity facilitated tissue integration and vascularization at the construct-recipient site. However, the interaction between PDLF and AO could have interfered with osteogenesis at the interface of soft and mineralizing tissues. Conclusions: Both matrices supported PDLF and AO attachment and proliferation in vitro. The membrane-scaffold construct facilitated tissue growth and vascularization while providing strength and form in vivo. | |
dc.source | Scopus | |
dc.subject | Alveolar osteoblasts | |
dc.subject | Graft construct | |
dc.subject | Human periodontal ligament fibroblasts | |
dc.subject | Poly (ε-caprolactone) | |
dc.type | Article | |
dc.contributor.department | BIOENGINEERING | |
dc.contributor.department | RESTORATIVE DENTISTRY | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.sourcetitle | International Journal of Oral and Maxillofacial Implants | |
dc.description.volume | 21 | |
dc.description.issue | 4 | |
dc.description.page | 526-534 | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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