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Title: Tissue engineering of a periodontal ligament-alveolar bone graft construct
Authors: Chou, A.M.
Sae-Lim, V. 
Hutmacher, D.W. 
Lim, T.M. 
Keywords: Alveolar osteoblasts
Graft construct
Human periodontal ligament fibroblasts
Poly (ε-caprolactone)
Issue Date: Jul-2006
Citation: Chou, A.M.,Sae-Lim, V.,Hutmacher, D.W.,Lim, T.M. (2006-07). Tissue engineering of a periodontal ligament-alveolar bone graft construct. International Journal of Oral and Maxillofacial Implants 21 (4) : 526-534. ScholarBank@NUS Repository.
Abstract: Purpose: This paper reports on a 2-phase study of a novel membrane-scaffold graft construct, its ability to support periodontal ligament fibroblast (PDLF) and alveolar osteoblast (AO) growth in vitro, and its use for tissue engineering a PDL-AO interface in vivo. Materials and Methods: Human PDLFs were seeded onto perforated poly(ε-caprolactone) membranes (n = 30) at 78,000 cells/cm 2; human AOs were seeded on poly(ε-caprolactone) scaffolds (n = 30) with fibrin glue at 625,000 cells/cm3. Cell attachment, morphology, viability, and metabolic activity were monitored for 3 weeks in vitro. Subsequently, cell-seeded membrane-scaffold constructs (experimental group, n = 9) and nonseeded constructs (control group, n = 4) assembled with fibrin glue were implanted subcutaneously into 7 athymic mice for 4 weeks. Results: PDLFs formed confluent layers on membranes, whereas AOs produced mineralized matrices within scaffolds upon osteoinduction in vitro. Well-vascularized tissue formation was observed after implantation. Integration at the membrane-scaffold interface was enhanced in the experimental group. Type I collagen, type III collagen, fibronectin, and vitronectin were found adjacent to membranes and within constructs. Bone sialoprotein expression and bone formation were undetectable. Discussion: Membrane perforation and scaffold porosity facilitated tissue integration and vascularization at the construct-recipient site. However, the interaction between PDLF and AO could have interfered with osteogenesis at the interface of soft and mineralizing tissues. Conclusions: Both matrices supported PDLF and AO attachment and proliferation in vitro. The membrane-scaffold construct facilitated tissue growth and vascularization while providing strength and form in vivo.
Source Title: International Journal of Oral and Maxillofacial Implants
ISSN: 08822786
Appears in Collections:Staff Publications

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