Silole nanocrystals as novel biolabels
Chan, C.P.-Y. Haeussler, M. Ben, Z.T. Dong, Y. Sin, K.-K. Mak, W.-C. Trau, D. Seydack, M. Renneberg, R.
Chan, C.P.-Y.
Haeussler, M.
Ben, Z.T.
Dong, Y.
Sin, K.-K.
Mak, W.-C.
Seydack, M.
Renneberg, R.
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Alternative Title
Abstract
A novel class of biofunctional silole nanocrystals with the potential to create highly sensitive immunoassay was firstly demonstrated. Biolabels were constructed by encapsulating nanocrystalline hexaphenylsilole [Ph 2Si(CPh)4; HPS] within ultrathin polyelectrolyte layers via the layer-by-layer (LbL) technique that provided an "interface" for the attachment of antibodies. A high ratio of fluorescent dyes to biomolecules (F/P ratio; 2.4×103) was achieved without self-quenching problem. The aggregation-induced emission (AIE) feature offered silole biolabels the sensitivity 40- to 140-fold higher than that of a start-of-the-art immunoassay using directly fluorescent-labeled antibodies. © 2004 Elsevier B.V. All rights reserved.
Keywords
Aggregation-induced emission (AIE), Biolabel, Fluorescence immunoassay (FIA), Layer-by-layer (LbL) technique, Silole
Source Title
Journal of Immunological Methods
Publisher
Series/Report No.
Collections
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Date
2004-12
DOI
10.1016/j.jim.2004.09.016
Type
Article