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https://doi.org/10.1016/j.jim.2004.09.016
| Title: | Silole nanocrystals as novel biolabels | Authors: | Chan, C.P.-Y. Haeussler, M. Ben, Z.T. Dong, Y. Sin, K.-K. Mak, W.-C. Trau, D. Seydack, M. Renneberg, R. |
Keywords: | Aggregation-induced emission (AIE) Biolabel Fluorescence immunoassay (FIA) Layer-by-layer (LbL) technique Silole |
Issue Date: | Dec-2004 | Citation: | Chan, C.P.-Y., Haeussler, M., Ben, Z.T., Dong, Y., Sin, K.-K., Mak, W.-C., Trau, D., Seydack, M., Renneberg, R. (2004-12). Silole nanocrystals as novel biolabels. Journal of Immunological Methods 295 (1-2) : 111-118. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jim.2004.09.016 | Abstract: | A novel class of biofunctional silole nanocrystals with the potential to create highly sensitive immunoassay was firstly demonstrated. Biolabels were constructed by encapsulating nanocrystalline hexaphenylsilole [Ph 2Si(CPh)4; HPS] within ultrathin polyelectrolyte layers via the layer-by-layer (LbL) technique that provided an "interface" for the attachment of antibodies. A high ratio of fluorescent dyes to biomolecules (F/P ratio; 2.4×103) was achieved without self-quenching problem. The aggregation-induced emission (AIE) feature offered silole biolabels the sensitivity 40- to 140-fold higher than that of a start-of-the-art immunoassay using directly fluorescent-labeled antibodies. © 2004 Elsevier B.V. All rights reserved. | Source Title: | Journal of Immunological Methods | URI: | http://scholarbank.nus.edu.sg/handle/10635/88124 | ISSN: | 00221759 | DOI: | 10.1016/j.jim.2004.09.016 |
| Appears in Collections: | Staff Publications |
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