Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jim.2004.09.016
Title: Silole nanocrystals as novel biolabels
Authors: Chan, C.P.-Y.
Haeussler, M.
Ben, Z.T.
Dong, Y.
Sin, K.-K.
Mak, W.-C.
Trau, D. 
Seydack, M.
Renneberg, R.
Keywords: Aggregation-induced emission (AIE)
Biolabel
Fluorescence immunoassay (FIA)
Layer-by-layer (LbL) technique
Silole
Issue Date: Dec-2004
Source: Chan, C.P.-Y., Haeussler, M., Ben, Z.T., Dong, Y., Sin, K.-K., Mak, W.-C., Trau, D., Seydack, M., Renneberg, R. (2004-12). Silole nanocrystals as novel biolabels. Journal of Immunological Methods 295 (1-2) : 111-118. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jim.2004.09.016
Abstract: A novel class of biofunctional silole nanocrystals with the potential to create highly sensitive immunoassay was firstly demonstrated. Biolabels were constructed by encapsulating nanocrystalline hexaphenylsilole [Ph 2Si(CPh)4; HPS] within ultrathin polyelectrolyte layers via the layer-by-layer (LbL) technique that provided an "interface" for the attachment of antibodies. A high ratio of fluorescent dyes to biomolecules (F/P ratio; 2.4×103) was achieved without self-quenching problem. The aggregation-induced emission (AIE) feature offered silole biolabels the sensitivity 40- to 140-fold higher than that of a start-of-the-art immunoassay using directly fluorescent-labeled antibodies. © 2004 Elsevier B.V. All rights reserved.
Source Title: Journal of Immunological Methods
URI: http://scholarbank.nus.edu.sg/handle/10635/88124
ISSN: 00221759
DOI: 10.1016/j.jim.2004.09.016
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