Please use this identifier to cite or link to this item: https://doi.org/10.1002/adma.200601169
Title: Micropatterning of proteins on 3D porous polymer films fabricated by using the breath-figure method
Authors: Zhang, Y. 
Wang, C. 
Issue Date: 6-Apr-2007
Source: Zhang, Y., Wang, C. (2007-04-06). Micropatterning of proteins on 3D porous polymer films fabricated by using the breath-figure method. Advanced Materials 19 (7) : 913-916. ScholarBank@NUS Repository. https://doi.org/10.1002/adma.200601169
Abstract: A porous film with a well-ordered pore structure, which was used as a template to pattern proteins, was prepared by using breadth-figure method. The linear amphiphilic PS-mNH2 functioned as a surfactant for reducing the surface tension at the interfaces between water droplets and PS and therefore increasing the contact surface, resulting in smaller water droplets forming in the polystyrene (PS). Fluorescein isothiocyanate (FITC) staining was used to confirm the presence of amino groups on the pore surface, because FITC has the ability to react with amino groups and the fluorescence can be observed using a fluorescence microscope. Ring-shaped fluorescence patterns were observed around the pores, but not observed in other regions of the film, indicating that the fluorescent FITC was attached to the pore surface. It was observed that the method can be used for 3D protein micropatterning with possible applications in tissue engineering.
Source Title: Advanced Materials
URI: http://scholarbank.nus.edu.sg/handle/10635/87924
ISSN: 09359648
DOI: 10.1002/adma.200601169
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