Please use this identifier to cite or link to this item: https://doi.org/10.1039/b909305b
Title: Fabrication of inflated LbL microcapsules with a 'bead-in-a-capsule' morphology
Authors: Bai, J.
Beyer, S.
Mak, W.C.
Trau, D. 
Issue Date: 2009
Citation: Bai, J., Beyer, S., Mak, W.C., Trau, D. (2009). Fabrication of inflated LbL microcapsules with a 'bead-in-a-capsule' morphology. Soft Matter 5 (21) : 4152-4160. ScholarBank@NUS Repository. https://doi.org/10.1039/b909305b
Abstract: The fabrication of inflated Layer-by-Layer (LbL) microcapsules with a unique 'bead-in-a-capsule' morphology is presented. Currently, the fabrication of LbL microcapsules using conventional aqueous LbL techniques usually results in microcapsules with a two-phase system (LbL capsular wall with an air, liquid, solid or hydrogel core). Here, we present the fabrication of inflated LbL microcapsules with a unique three-phase system (LbL capsular wall, hydrogel microbead in an aqueous core) by using the Reverse-Phase LbL (RP-LbL) technique. The RP-LbL technique is performed in an organic solvent and allows encapsulation of water-soluble templates and molecules with high efficiency. Firstly, the RP-LbL technique is used to coat polymer layers onto agarose microbeads containing TRIS buffer for the formation of LbL capsular walls onto the microbeads and to minimize out-diffusion of encapsulated TRIS. Next, the polymer-coated agarose microbeads are transferred from an organic to an aqueous solvent where the TRIS molecules induce an osmotic pressure in the microcapsules' interior. This pressure drives the inflation of the LbL microcapsules that causes the expansion of the LbL capsular walls. Fluorescence staining reveals that the inflated LbL microcapsules consist of an agarose microbead suspended within the aqueous interior of the capsule but still attached to the LbL capsular wall at one point; thereby displaying a 'bead-in-a-capsule' morphology. It was demonstrated that the degree of inflation depends on the concentration of pre-loaded TRIS and the number of coated polymer layers. Also, ADOGEN® 464 (a cationic surfactant) is required for the fabrication of the inflated LbL microcapsules. The mass of dextran macromolecules (65-2000 kDa) diffusing through the LbL capsular wall had decreased by at least 49% after expansion of the capsular wall. Inflated microcapsules were shown to be capable of controlling the distribution of two different materials internally. Hence, it is possible that inflated microcapsules can permit localized control over chemical or enzymatic reactions for future uses in biomedical applications. © 2009 The Royal Society of Chemistry.
Source Title: Soft Matter
URI: http://scholarbank.nus.edu.sg/handle/10635/87768
ISSN: 1744683X
DOI: 10.1039/b909305b
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

14
checked on Sep 18, 2018

WEB OF SCIENCETM
Citations

13
checked on Sep 18, 2018

Page view(s)

27
checked on Jun 8, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.