Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2013.12.060
Title: Bacterial imaging with photostable upconversion fluorescent nanoparticles
Authors: Ong, L.C.
Ang, L.Y. 
Alonso, S.
Zhang, Y. 
Keywords: Bacteria labeling
Escherichia coli
Fluorescence imaging
Upconversion nanoparticles
Issue Date: Mar-2014
Citation: Ong, L.C., Ang, L.Y., Alonso, S., Zhang, Y. (2014-03). Bacterial imaging with photostable upconversion fluorescent nanoparticles. Biomaterials 35 (9) : 2987-2998. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2013.12.060
Abstract: Autofluorescence, photodamage and photobleaching are often encountered when using downconverting fluorophores and fluorescent proteins for bacteria labeling. These caveats represent a serious limitation when trying to map bacteria dissemination for prolonged periods. Upconversion nanoparticles (UCNs), which are able to convert low energy near-infrared (NIR) excitation light into higher energy visible or NIR light, can address these limitations. These particles' unique optical properties translate into attractive advantages of minimal autofluorescence, reduced photodamage, deeper tissue penetration and prolonged photostability. Here, we report a UCN-based bacteria labeling strategy using Escherichia coli as prototypic bacteria. A comparative analysis highlighted the superior photostability of UCN-labeled bacteria over green fluorescent protein-expressing bacteria. Infection study of UCN-labeled bacteria in dendritic cells indicated co-localization of the UCN signal with bacterial position for up to 6h post-infection. Furthermore, long-term monitoring of the same infected cells demonstrated the potential to utilize photostable UCN-based imaging for bacterial trafficking purposes. © 2013 Elsevier Ltd.
Source Title: Biomaterials
URI: http://scholarbank.nus.edu.sg/handle/10635/87717
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2013.12.060
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

39
checked on Dec 11, 2018

WEB OF SCIENCETM
Citations

37
checked on Dec 11, 2018

Page view(s)

87
checked on Dec 7, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.