Please use this identifier to cite or link to this item:
|Title:||Gene delivery to the heart by magnetic nanobeads|
|Citation:||Li, W., Nesselmann, C., Zhou, Z., Ong, L.-L., Öri, F., Tang, G., Kaminski, A., Lützow, K., Lendlein, A., Liebold, A., Stamm, C., Wang, J., Steinhoff, G., Ma, N. (2007-04). Gene delivery to the heart by magnetic nanobeads. Journal of Magnetism and Magnetic Materials 311 (1 SPEC. ISS.) : 336-341. ScholarBank@NUS Repository.|
|Abstract:||Gene delivery with non-viral gene vectors to the cardiovascular system suffers from low transfection efficiency. In this study, magnetic fields were investigated to assist cardiovascular gene delivery via magnetic nanobeads both in vitro and in vivo. The magnetic field was provided with a 1120 mT Nd-Fe-B permanent magnet while complexes of poly-ethyleneimine (PEI) and various DNA plasmids were conjugated with magnetic nanobeads (MNB) using a Sulfo-NHS-LC-Biotin linker. In vitro results showed that transfection in two cell lines was 30-80-fold higher in magnetically conjugated MNB/PEI/DNA complexes than transfection from PEI/DNA complexes alone. Similarly, in vivo results using mouse models showed 72 h after injection observable gene expression in the heart with conjugated MNB/PEI/DNA complexes, but barely with PEI/DNA complexes alone. © 2006 Elsevier B.V. All rights reserved.|
|Source Title:||Journal of Magnetism and Magnetic Materials|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 27, 2018
WEB OF SCIENCETM
checked on Apr 25, 2018
checked on Jun 1, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.