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|Title:||A novel amperometric biosensor based on ZnO:Co nanoclusters for biosensing glucose|
|Citation:||Zhao, Z.W., Chen, X.J., Tay, B.K., Chen, J.S., Han, Z.J., Khor, K.A. (2007-08-30). A novel amperometric biosensor based on ZnO:Co nanoclusters for biosensing glucose. Biosensors and Bioelectronics 23 (1) : 135-139. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2007.03.014|
|Abstract:||ZnO:Co nanoclusters were synthesized by nanocluster-beam deposition with averaged particle size of 5 nm and porous structure, which were for the first time adopted to construct a novel amperometric glucose biosensor. Glucose oxidase was immobilized into the ZnO:Co nanocluster-assembled thin film through Nafion-assisted cross-linking technique. Due to the high specific active sites and high electrocatalytic activity of the ZnO:Co nanoclusters, the constructed glucose biosensor showed a high sensitivity of 13.3 μA/mA cm2. The low detection limit was estimated to be 20 μM (S/N = 3) and the apparent Michaelis-Menten constant was found to be 21 mM, indicating the high affinity of the enzyme on ZnO:Co nanoclusters to glucose. The results show that the ZnO:Co nanocluster-assembled thin films with nanoporous structure and nanocrystallites have potential applications as platforms to immobilize enzyme in biosensors. © 2007 Elsevier B.V. All rights reserved.|
|Source Title:||Biosensors and Bioelectronics|
|Appears in Collections:||Staff Publications|
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