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Title: In Vivo Study of ACL Regeneration Using Silk Scaffolds In a Pig Model
Authors: Liu, H.
Fan, H.
Toh, S.L. 
Goh, J.C.H. 
Keywords: ACL
Pig model
Silk scaffold
Issue Date: 2009
Citation: Liu, H.,Fan, H.,Toh, S.L.,Goh, J.C.H. (2009). In Vivo Study of ACL Regeneration Using Silk Scaffolds In a Pig Model. IFMBE Proceedings 23 : 1512-1514. ScholarBank@NUS Repository.
Abstract: Although most in vitro studies indicate that silk is a suitable biomaterial for ligament tissue engineering, in vivo studies of implanted silk scaffolds for ligament reconstruction are still lacking, especially in a big animal. The objective of this study is to investigate anterior cruciate ligament (ACL) regeneration using mesenchymal stem cells (MSCs) and silk scaffold in a pig model. The scaffold was fabricated by incorporating microporous silk sponges into knitted silk mesh, which mimicked the structures of ligament extracellular matrix (ECM). In vitro culture already demonstrated that MSCs on scaffolds proliferated vigorously and produced abundant collagen. The MSCs/scaffold was implanted to regenerate ACL in a pig model. After 24 weeks, histology observation showed that MSCs were distributed throughout the regenerated ligament and exhibited fibroblast morphology. The key ligament ECM components including collagen I, collagen III, and tenascin-C were produced prominently. The tensile strength of regenerated ligament also met the mechanical requirements. In conclusion, the results imply that silk scaffold has great potentials in future clinical applications.
Source Title: IFMBE Proceedings
ISBN: 9783540928409
ISSN: 16800737
DOI: 10.1007/978-3-540-92841-6_374
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