Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2006.06.017
Title: Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells
Authors: Chua, K.-N.
Chai, C.
Lee, P.-C.
Tang, Y.-N.
Ramakrishna, S. 
Leong, K.W.
Mao, H.-Q.
Keywords: Electrospinning
Expansion
Hematopoietic stem cells
Nanofiber
Stem cell therapy
Issue Date: Dec-2006
Citation: Chua, K.-N., Chai, C., Lee, P.-C., Tang, Y.-N., Ramakrishna, S., Leong, K.W., Mao, H.-Q. (2006-12). Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells. Biomaterials 27 (36) : 6043-6051. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2006.06.017
Abstract: Interaction between hematopoietic stem/progenitor cells (HSPCs) and their extra cellular matrix components is an integral part of the signaling control for HSPC survival, proliferation and differentiation. We hypothesized that both substrate topographical cues and biochemical cues could act synergistically with cytokine supplementation to improve ex vivo expansion of HSPCs. In this study, we compared the ex vivo expansion of human umbilical cord blood CD34+ cells on unmodified, hydroxylated, carboxylated and aminated nanofibers and films. Results from 10-day expansion cultures showed that aminated nanofiber mesh and film were most efficient in supporting the expansion of the CD34+CD45+ cells (195-fold and 178-fold, respectively), as compared to tissue culture polystyrene (50-fold, p
Source Title: Biomaterials
URI: http://scholarbank.nus.edu.sg/handle/10635/85711
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2006.06.017
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