Please use this identifier to cite or link to this item:
|Title:||Biocompatibility evaluation of electrically conductive nanofibrous scaffolds for cardiac tissue engineering|
|Source:||Kai, D., Prabhakaran, M.P., Jin, G., Ramakrishna, S. (2013-05-07). Biocompatibility evaluation of electrically conductive nanofibrous scaffolds for cardiac tissue engineering. Journal of Materials Chemistry B 1 (17) : 2305-2314. ScholarBank@NUS Repository. https://doi.org/10.1039/c3tb00151b|
|Abstract:||Myocardial tissue engineering offers a novel technology to improve or regenerate cardiac functions using a combination of cells, biomaterials and engineering strategies. Inspired by low-resistance pathways for electrical signal propagation in the native heart tissue, electrically conductive nanofibrous scaffolds composed of melanin, poly(l-lactide-co-ε- caprolactone) and gelatin were fabricated to provide electrophysiological cues to cardiac myocytes and mimic the native myocardial environment. Our results show that by increasing the concentration of melanin to 40% within the composite, the fiber diameters reduced to 153 ± 30 nm, modulus decreased to 7.1 ± 0.6 MPa, and conductance increased to 259.51 ± 187.60 μS cm-1. Results of cell proliferation and immunostaining analysis of human cardiac myocytes demonstrated that the conductive nanofibers containing 10% melanin promote cell interaction with expression of cardiac-specific proteins compared to other scaffolds. Electrical stimulation through the scaffolds showed enhanced cell proliferation and the expression of connexin-43, signifying the potential of using melanin containing nanofibers as a suitable cardiac patch for the regeneration of infarct myocardium. This journal is © The Royal Society of Chemistry.|
|Source Title:||Journal of Materials Chemistry B|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Mar 7, 2018
WEB OF SCIENCETM
checked on Nov 15, 2017
checked on Apr 20, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.