Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2010.07.097
Title: A comparison of bioreactors for culture of fetal mesenchymal stem cells for bone tissue engineering
Authors: Zhang, Z.-Y. 
Teoh, S.H. 
Teo, E.Y. 
Khoon Chong, M.S.
Shin, C.W.
Tien, F.T.
Choolani, M.A.
Chan, J.K.Y.
Keywords: Bioreactor
Bone tissue engineering
Mesenchymal stem cells
Micro CT
Issue Date: Nov-2010
Source: Zhang, Z.-Y., Teoh, S.H., Teo, E.Y., Khoon Chong, M.S., Shin, C.W., Tien, F.T., Choolani, M.A., Chan, J.K.Y. (2010-11). A comparison of bioreactors for culture of fetal mesenchymal stem cells for bone tissue engineering. Biomaterials 31 (33) : 8684-8695. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2010.07.097
Abstract: Bioreactors provide a dynamic culture system for efficient exchange of nutrients and mechanical stimulus necessary for the generation of effective tissue engineered bone grafts (TEBG). We have shown that biaxial rotating (BXR) bioreactor-matured human fetal mesenchymal stem cell (hfMSC) mediated-TEBG can heal a rat critical sized femoral defect. However, it is not known whether optimal bioreactors exist for bone TE (BTE) applications. We systematically compared this BXR bioreactor with three most commonly used systems: Spinner Flask (SF), Perfusion and Rotating Wall Vessel (RWV) bioreactors, for their application in BTE. The BXR bioreactor achieved higher levels of cellularity and confluence (1.4-2.5x, p < 0.05) in large 785 mm3 macroporous scaffolds not achieved in the other bioreactors operating in optimal settings. BXR bioreactor-treated scaffolds experienced earlier and more robust osteogenic differentiation on von Kossa staining, ALP induction (1.2-1.6×, p < 0.01) and calcium deposition (1.3-2.3×, p < 0.01). We developed a Micro CT quantification method which demonstrated homogenous distribution of hfMSC in BXR bioreactor-treated grafts, but not with the other three. BXR bioreactor enabled superior cellular proliferation, spatial distribution and osteogenic induction of hfMSC over other commonly used bioreactors. In addition, we developed and validated a non-invasive quantitative micro CT-based technique for analyzing neo-tissue formation and its spatial distribution within scaffolds. © 2010 Elsevier Ltd.
Source Title: Biomaterials
URI: http://scholarbank.nus.edu.sg/handle/10635/84773
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2010.07.097
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

56
checked on Feb 20, 2018

WEB OF SCIENCETM
Citations

54
checked on Nov 22, 2017

Page view(s)

31
checked on Feb 15, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.