Please use this identifier to cite or link to this item:
|Title:||Optimization of agitation, aeration, and temperature conditions for maximum β-mannanase production|
|Authors:||Feng, Y. |
|Citation:||Feng, Y., He, Z., Ong, S.L., Hu, J., Zhang, Z., Ng, W.J. (2003-02-03). Optimization of agitation, aeration, and temperature conditions for maximum β-mannanase production. Enzyme and Microbial Technology 32 (2) : 282-289. ScholarBank@NUS Repository. https://doi.org/10.1016/S0141-0229(02)00287-9|
|Abstract:||The effects of cultivation temperature, aeration rate, and agitation speed on the production of β-mannanase by Bacillus licheniformis NK-27 in a batch fermenter were investigated in this study. Results revealed that temperature was the most significant factor in terms of its effect on β-mannanase production. It influenced β-mannanase production by affecting the other parameters including bacteria growth, pH, dissolved oxygen, total and reducing sugars. Agitation speed and aeration rate could affect dissolved oxygen concentration which in turn affected cell growth and β-mannanase production. A maximum β-mannanase activity of 212.0Uml-1 was attained in 36h of cultivation when aeration rate, agitation speed, and temperature were controlled at 0.75vvm, 600rpm, and 30°C, respectively. The maximum β-mannanase activity in the fermenter was close to that obtained from the shake flask fermentation study (198.2Uml-1). However, the duration of fermentation cycle in the fermenter study was shorter than the corresponding duration obtained from the shake flask experiment by 12h. © 2002 Elsevier Science Inc. All rights reserved.|
|Source Title:||Enzyme and Microbial Technology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Aug 15, 2018
WEB OF SCIENCETM
checked on Jul 23, 2018
checked on May 18, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.