Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/80240
Title: STUDYING THE ROLE OF BIOCHEMICAL CUES IN IMPROVING HUMAN MESENCHYMAL STEM CELL BASED CARTILAGE TISSUE ENGINEERING USING FIBROUS POLYELECTROLYTE HYDROGELS
Authors: DEEPAK RAGHOTHAMAN
Keywords: Articular cartilage, chondrogenesis, extra-cellular matrix, hydrogels, mesenchymal stem cells, tissue engineering
Issue Date: 22-Jan-2014
Citation: DEEPAK RAGHOTHAMAN (2014-01-22). STUDYING THE ROLE OF BIOCHEMICAL CUES IN IMPROVING HUMAN MESENCHYMAL STEM CELL BASED CARTILAGE TISSUE ENGINEERING USING FIBROUS POLYELECTROLYTE HYDROGELS. ScholarBank@NUS Repository.
Abstract: As cell-cell and cell-matrix interactions are crucial for chondrogenic differentiation, biomimetic scaffolds promoting such events were developed to improve stem cell based cartilage tissue engineering. Chitin-alginate hydrogels fabricated by interfacial polyelectrolyte complexation (IPC) were employed for presenting various biochemical cues to human bone marrow derived mesenchymal stem cells (MSC) and chondrocytes. Such studies found that: (i) submicron alignment of collagen type I (Col I) in IPC hydrogel promoted superior chondrogenesis of MSC , in comparison with IPC-no cue (without Col I) and conventional Col I hydrogels, by facilitating robust cell-cell, cell-matrix interactions; (ii) biochemical matrix cues such as Col I, Chondroitin sulfate and hyaluronic acid were found to direct MSC chondrogenesis to different zonal phenotypes of articular cartilage; and (iii) a differential preference of matrix microenvironments was elucidated between MSC and chondrocyte in deriving neocartilage. These studies illustrated the potential of IPC based biomimetic strategies to augment cartilage tissue engineering.
URI: http://scholarbank.nus.edu.sg/handle/10635/80240
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