Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/79982
Title: GENERATING VIRUS-RESISTANT TRANSGENIC PLANTS HARBOURING ARTIFICIAL MICRORNA CONTAINING COMBINED SEQUENCES FROM TWO ORCHID VIRUSES
Authors: XIE ZHICHENG
Keywords: artificial microRNA, CymMV, ORSV, virus resistance, transgenic, orchids
Issue Date: 6-May-2014
Source: XIE ZHICHENG (2014-05-06). GENERATING VIRUS-RESISTANT TRANSGENIC PLANTS HARBOURING ARTIFICIAL MICRORNA CONTAINING COMBINED SEQUENCES FROM TWO ORCHID VIRUSES. ScholarBank@NUS Repository.
Abstract: ARTIFICIAL MICRORNAS BASED ON RNA-DEPENDENT RNA POLYMERASE OF CYMBIDIUM MOSAIC VIRUS (CYMMV) AND ODONTOGLOSSUM RINGSPOT VIRUS (ORSV) WERE DESIGNED AND CONSTRUCTED INTO A SINGLE TRANSGENE. FROM THE RESULTS OBTAINED, THE T2 TRANSGENIC NICOTIANA BENTHAMIANA PLANTS DID NOT SHOW ANY TYPICAL VIRUS DISEASE SYMPTOMS 21 DAYS POST INOCULATION AND ABSENCE OF VIRAL COAT PROTEIN IN THE PLANT CELL. ATTEMPTS WERE MADE TO GENERATE TRANSGENIC DENDROBIUM CHAO PRAYA SMILE (DCPS) BY USING PROTOCORMS THROUGH AGROBACTERIUM-MEDIATED TRANSFORMATION (AMT). DUE TO ORCHID SLOW GROWTH RATE, THE REMAINING PUTATIVE TRANSGENIC ORCHIDS ARE STILL SURVIVING ON SELECTION MEDIUM AND THIS IS AN INDICATION THAT THE TRANSGENIC ORCHIDS COULD BE PRODUCE AND THUS MAY POSSESS THE ABILITY TO RESIST AGAINST CYMMV AND ORSV INFECTION. THE SUITABILITY OF POLLINIA FROM DCPS AS STARTING MATERIALS IN ORCHID TRANSFORMATION WAS INVESTIGATED. THE PRELIMINARY RESULTS SUGGESTED THAT THERE IS POTENTIAL OF USING POLLINIA AS STARTING MATERIALS
URI: http://scholarbank.nus.edu.sg/handle/10635/79982
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