Please use this identifier to cite or link to this item:
|Title:||Relationship between cell function and initial cell seeding density of primary porcine chondrocytes in vitro|
|Source:||Zhang, K.,Wang, L.,Han, Q.,Heng, B.C.,Yang, Z.,Ge, Z. (2013-10). Relationship between cell function and initial cell seeding density of primary porcine chondrocytes in vitro. Biomedical Engineering - Applications, Basis and Communications 25 (5) : -. ScholarBank@NUS Repository. https://doi.org/10.4015/S1016237213400012|
|Abstract:||Maintenance of differentiated functional phenotype within in vitro chondrocyte culture requires seeding at high densities with large numbers of cells. However, optimal cell seeding numbers and densities remain elusive due to multiple varying parameters and different methodologies utilized in previous studies. In the current study, we tried to investigate the relationship between cell seeding number and differentiated functional phenotype of in vitro cultured chondrocytes. Varying numbers of primary porcine chondrocytes (0.25, 2.5, 25 and 250 K) were seeded in 96 well-plates and cultured for 4 weeks. Cell proliferation, glycosaminoglycan (GAG) production and gene expression levels of Sox9, aggrecan, COL II and COL I were evaluated. The results showed that GAG content was high in the 0.25 and 25 K groups, gene expression of Sox9 was high in the 2.5, 25 and 250 K groups and expression of COL II was high in the 25 K group, whereas expression of COL I was low in the 0.25, 25 and 250 K groups. It is concluded that the seeding number and density of the 25 K (78 K cells/cm 2) group achieved the optimal balance between functional phenotype of individual cells and the total ECM production for in vitro cultured chondrocytes. © 2013 National Taiwan University.|
|Source Title:||Biomedical Engineering - Applications, Basis and Communications|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Mar 7, 2018
checked on Mar 9, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.