Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.tice.2005.03.008
Title: Osteogenic differentiation within intact human embryoid bodies result in a marked increase in osteocalcin secretion after 12 days of in vitro culture, and formation of morphologically distinct nodule-like structures
Authors: Cao, T. 
Heng, B.C. 
Ye, C.P.
Liu, H. 
Toh, W.S. 
Robson, P.
Li, P.
Hong, Y.H. 
Stanton, L.W.
Keywords: Differentiation
Embryonic
Human
Osteocalcin
Osteogenic
Stem cells
Issue Date: Aug-2005
Citation: Cao, T., Heng, B.C., Ye, C.P., Liu, H., Toh, W.S., Robson, P., Li, P., Hong, Y.H., Stanton, L.W. (2005-08). Osteogenic differentiation within intact human embryoid bodies result in a marked increase in osteocalcin secretion after 12 days of in vitro culture, and formation of morphologically distinct nodule-like structures. Tissue and Cell 37 (4) : 325-334. ScholarBank@NUS Repository. https://doi.org/10.1016/j.tice.2005.03.008
Abstract: Osteogenic lineages derived from human embryonic stem cells hold much promise for clinical application in bone regeneration, in addition to providing a useful research model in developmental biology, and for pharmacological and cytotoxicity screening of bone-related biomaterials and drugs in vitro. Previously, osteogenic differentiation of human embryonic stem cells was achieved through dissociation of embryoid bodies by trypsinization, prior to culture with osteogenesis-promoting medium. This study therefore attempted a new approach: That is to achieve osteogenesis within intact human embryoid bodies. After 22 days of culture in osteogenesis-promoting medium comprising a cocktail of accorbic acid, β-glycerophosphate and dexamethasone, the attached embryoid bodies exhibited much cellular outgrowth and migration, and formed morphologically distinct nodule-like structures. These were somewhat similar to osteogenic nodules formed by mesenchymal stem cells, as reported by previous studies. Immunohistochemical staining and RT-PCR analysis confirmed the presence of osteogenic cells within these nodule-like structures. Additionally, the quantitative assay of osteocalcin secretion demonstrated a rapid sharp increase in osteocalcin expression on day 12 of in vitro culture, which could suggest the appearance of differentiated osteoblasts from day 12 onwards. Future work will attempt to investigate whether other cytokines, growth factors and chemical compounds could further enhance osteogenesis within intact human embryoid bodies. © 2005 Elsevier Ltd. All rights reserved.
Source Title: Tissue and Cell
URI: http://scholarbank.nus.edu.sg/handle/10635/79972
ISSN: 00408166
DOI: 10.1016/j.tice.2005.03.008
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

56
checked on Jun 14, 2018

WEB OF SCIENCETM
Citations

52
checked on May 1, 2018

Page view(s)

57
checked on Feb 25, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.