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|Title:||Enamel diffusion modulated by Er:YAG laser. (Part 1)-FRAP|
|Citation:||Maung, N.L., Wohland, T., Hsu, C.-Y.S. (2007-10). Enamel diffusion modulated by Er:YAG laser. (Part 1)-FRAP. Journal of Dentistry 35 (10) : 787-793. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jdent.2007.07.011|
|Abstract:||Several studies have demonstrated the caries protective effect of lasers by strengthening enamel crystalline structure. However, the effect of laser on enamel diffusion (ED) remains unclear. Objectives: This study aimed to quantify the laser-induced alteration of diffusion coefficients (DC) in enamel using fluorescence recovery after photobleaching (FRAP). Methods: Eleven caries-free enamel sections were characterized morphologically using stereomicroscopy, polarized light microscopy and scanning electron microscopy, before and after laser treatment with Er:YAG laser 50 mJ × 5 s × 5 Hz. With 20 μM fluorescein, DCs were measured (n = 11) by FRAP coupled with confocal microscopy. Results: The DCs measured were 2.89 ± 0.61 × 10-7 cm2/s and 4.076 ± 0.73 × 10-7 cm2/s, at the lased and unlased areas, respectively (p = 0.001). Conclusions: This study has confirmed the reduction of ED as a potential mechanism involved in laser-induced caries prevention. FRAP was demonstrated to be a promising technique for evaluating diffusion-related phenomenon in enamel. © 2007.|
|Source Title:||Journal of Dentistry|
|Appears in Collections:||Staff Publications|
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