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Title: Investigations on Rab31's Role in EGFR Trafficking and Neural Progenitor Cell Differentiation Towards Astroglia
Keywords: Rab GTPase, EGFR, NPC, trafficking, astroglial differentiation, cell biology
Issue Date: 16-Apr-2014
Citation: CHRISTELLE CHUA EN LIN (2014-04-16). Investigations on Rab31's Role in EGFR Trafficking and Neural Progenitor Cell Differentiation Towards Astroglia. ScholarBank@NUS Repository.
Abstract: We have investigated Rab31?s role in the trafficking of ligand-bound epidermal growth factor (EGF) receptor (EGFR) internalised through receptor-mediated endocytosis. Depletion of Rab31 inhibited, while Rab31 overexpression enhanced, EGFR trafficking to the late endosomes. Rab31 was found to interact with EGFR. Loss of early endosome antigen 1 (EEA1), a Rab31 effector, and GAPex5, a Rab31 guanine nucleotide exchange factor (GEF), reduced the interaction between Rab31 and EGFR. To explore the physiological role of Rab31 which is highly expressed in radial glia and mature astrocytes, we looked at Rab31 in neural progenitor cells (NPCs). When NPCs were induced to differentiate, Rab31 levels dipped then reappeared in a subset of the glial fibrillary acidic protein (GFAP)-positive astrocyte population. Depletion of Rab31 decreased the percentage of GFAP-positive cells obtained, suggesting that Rab31 plays a role in the differentiation of NPCs. Results presented in this thesis have implications for both our understanding of neurogenesis and cancer therapeutics.
Appears in Collections:Ph.D Theses (Open)

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