Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/78962
Title: An Asthma-Allergen Specific Animal Model for the Study of Responses to Dust Mite Allergen Induced Asthma
Authors: KENNETH WONG HOK SUM
Keywords: Asthma, T cells, Epitope mapping, House Dust mite, Blomia tropicalis, Allergy
Issue Date: 26-Dec-2013
Citation: KENNETH WONG HOK SUM (2013-12-26). An Asthma-Allergen Specific Animal Model for the Study of Responses to Dust Mite Allergen Induced Asthma. ScholarBank@NUS Repository.
Abstract: T cells play a central role in the pathogenesis of asthma. In the murine model of asthma, most of the studies were conducted with ovalbumin (OVA) as a surrogate allergen. In the OVA model of asthma, CD8 T cells attenuated the allergic inflammatory response. We established a murine model of house dust mite (HDM) induced asthma and adoptively transferred Der p 1 specific CD8 T cells into these mice. We showed that, unlike in the OVA model, CD8 T cells were unable to attenuate the allergic inflammatory responses. We also cloned the T cell receptors of the Der p 1 transgenic CD8 T cells for the generation of T cell receptor transgenic mice for future studies. We also mapped epitopes from Blo t 5 that were recognized by CD4 T cells. We showed that the epitope-specific CD4 T cells were recruited to the lungs following allergen challenge. Induction of regulatory response to the peptide by intradermal administration of these peptides reduced the severity of the allergic lung responses.
URI: http://scholarbank.nus.edu.sg/handle/10635/78962
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Thesis - WongKHS.pdf19.15 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

328
checked on Oct 20, 2018

Download(s)

176
checked on Oct 20, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.