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Title: | An Asthma-Allergen Specific Animal Model for the Study of Responses to Dust Mite Allergen Induced Asthma | Authors: | KENNETH WONG HOK SUM | Keywords: | Asthma, T cells, Epitope mapping, House Dust mite, Blomia tropicalis, Allergy | Issue Date: | 26-Dec-2013 | Citation: | KENNETH WONG HOK SUM (2013-12-26). An Asthma-Allergen Specific Animal Model for the Study of Responses to Dust Mite Allergen Induced Asthma. ScholarBank@NUS Repository. | Abstract: | T cells play a central role in the pathogenesis of asthma. In the murine model of asthma, most of the studies were conducted with ovalbumin (OVA) as a surrogate allergen. In the OVA model of asthma, CD8 T cells attenuated the allergic inflammatory response. We established a murine model of house dust mite (HDM) induced asthma and adoptively transferred Der p 1 specific CD8 T cells into these mice. We showed that, unlike in the OVA model, CD8 T cells were unable to attenuate the allergic inflammatory responses. We also cloned the T cell receptors of the Der p 1 transgenic CD8 T cells for the generation of T cell receptor transgenic mice for future studies. We also mapped epitopes from Blo t 5 that were recognized by CD4 T cells. We showed that the epitope-specific CD4 T cells were recruited to the lungs following allergen challenge. Induction of regulatory response to the peptide by intradermal administration of these peptides reduced the severity of the allergic lung responses. | URI: | http://scholarbank.nus.edu.sg/handle/10635/78962 |
Appears in Collections: | Ph.D Theses (Open) |
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