Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/78938
Title: DEVELOPING MONITORING TOOLS FOR TOMORROW?S INVASIVES: SPECIES LISTS, DNA BARCODES, AND IMAGES FOR ORNAMENTAL FISH
Authors: YI YOUGUANG
Keywords: DNA Barcod*, Aquarium fish*, Ornamental fish*, COI, Biological Invasion, monitoring
Issue Date: 31-Mar-2014
Citation: YI YOUGUANG (2014-03-31). DEVELOPING MONITORING TOOLS FOR TOMORROW?S INVASIVES: SPECIES LISTS, DNA BARCODES, AND IMAGES FOR ORNAMENTAL FISH. ScholarBank@NUS Repository.
Abstract: Ornamental fish are a major source of invasive species in freshwater habitats. In order to control and monitor introductions, it is important to know which species are in the trade and to develop identification tools for these species. Here I first study the species diversity in the trade by comparing two published lists with trade data for Singapore (2009-2011). I establish that a very large number of species (4769) are being traded, the lists and trade data are inconsistent, many species in Singapore?s trade are wild-caught, and that new species are continuously added. I then image and generate DNA barcodes for 1448 specimens belonging to 554 species of which 334 species had not previously been barcoded. The images are used to build an online image database for ornamental fish while the DNA barcodes are used for testing species-specificity at three levels; local, global, and systematic. First, I establish whether DNA barcodes can be used for identifying the 89 of the 105 freshwater fish species living in Singapore. An identification efficiency of 77% to 89% indicates that COI can be used to allocate specimen to species at an island scale. I then determine identification success rates of DNA barcodes at a global scale based on all my data and all available COI sequences in Genbank. An identification efficiency of 77% to 91% indicates that COI can be used to allocate specimen to species at a global scale. Lastly, I collaborate with colleagues in New Zealand to test whether DNA barcodes are diagnostic for cypriniform fishes. An identification efficiency of 90% to 99% is established for the 172 ornamental cyprinid fish species sampled. Results indicate that COI can be used effectively for identifying fish at local, global and systematic level.
URI: http://scholarbank.nus.edu.sg/handle/10635/78938
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