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Title: Dynamic studies of Type I and Type II cadherins EC domains
Authors: WU FEI
Keywords: cadherins,force spectroscopy, cell adhesion, atomic force microscopy, magnetic tweezers, steered molecular dynamics
Issue Date: 23-Jan-2014
Source: WU FEI (2014-01-23). Dynamic studies of Type I and Type II cadherins EC domains. ScholarBank@NUS Repository.
Abstract: Cadherin-mediated adhesion plays a crucial role in multicellular organisms. Dysfunction within this adhesion system has major consequences in many pathologies. However, mechanisms controlling cadherin recognition and adhesive strengthening are only partially understood. Here, we investigated the homophilic interactions and mechanical stability of the EC domains of Type I and Type II cadherins at the molecular level using AFM, magnetic tweezers and SMD. Besides exhibiting stronger homophilic interactions, Type I cadherins showed more efficient force-induced self-strengthening of interactions than Type II cadherins. In addition, the unbinding forces for both cadherins showed an overlap with unfolding forces, indicating that partial unfolding/deformation of the cadherin EC domains may take place before the rupture of their homophilic interactions. This conformational change may be involved in cadherins physiology function and may contribute to the significant differences in adhesive strength generated by specific mechanical weak points in type I and type II cadherins.
Appears in Collections:Ph.D Theses (Open)

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