Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/78679
Title: CHARACTERIZATION OF DNA METHYLATION PATTERNS IN HUMAN EMBRYONIC STEM CELLS AND COLORECTAL CANCER USING WHOLE GENOME BISULFITE SEQUENCING
Authors: WONG HUIJUN, ELEANOR
Keywords: Epigenetics, DNA methylation, Bisulfite sequencing, stem cells
Issue Date: 14-Aug-2013
Citation: WONG HUIJUN, ELEANOR (2013-08-14). CHARACTERIZATION OF DNA METHYLATION PATTERNS IN HUMAN EMBRYONIC STEM CELLS AND COLORECTAL CANCER USING WHOLE GENOME BISULFITE SEQUENCING. ScholarBank@NUS Repository.
Abstract: DNA methylation is an epigenetic modification essential for normal development. Inappropriate regulation of DNA methylation can cause abnormal silencing of tumor suppressor genes leading to cancer development. Despite the importance of this mechanism in human health, our existing knowledge is restricted to gene promoters and CpG islands (CGIs) which were thought to be key regulators of epigenetics. However, less than 7% of CpGs are located within the CGIs, many CpGs remain uninvestigated resulting in a highly fragmented view of the epigenome. Here, I applied whole genome bisulfite sequencing (WGB-seq) to comprehensively profile undifferentiated human embryonic stem (hES) cell and colon cancer methylome at single base resolution. I will present how an integrative analysis of DNA methylation, gene expression and histone modifications can identify the differentially methylated regions and genes involved in the regulation of cell differentiation and CRC.
URI: http://scholarbank.nus.edu.sg/handle/10635/78679
Appears in Collections:Ph.D Theses (Open)

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