Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/78638
Title: CHARACTERISATION OF HERVS IN AN ASIAN POPULATION: A SINGAPORE PERSPECTIVE
Authors: WOO WEE HONG
Keywords: HERV, colorectal cancer, carcinogeneis, prevalence, endogenous retrovirus, Singapore
Issue Date: 20-Jan-2014
Citation: WOO WEE HONG (2014-01-20). CHARACTERISATION OF HERVS IN AN ASIAN POPULATION: A SINGAPORE PERSPECTIVE. ScholarBank@NUS Repository.
Abstract: Human endogenous retroviruses (HERVs) are a family of viruses that constitute ~8% of the human genome. Interestingly, HERV-H is selectively expressed in colon cancers but not in normal tissues. As colorectal cancer is the most common cancer for both men and women in Singapore, we further investigate the role of HERV-H in colorectal carcinogenesis. We first demonstrated that the HERV-H gene is not lost from the Singapore population, and in fact, exists at high prevalence (89.3%) in the population sampled. We next established in vitro models to investigate HERV-H¿s role in neoplastic transformation. Our results demonstrated that HERV-H overexpression augmented cell proliferation, motility, ability to form cancer stem cell-like colonospheres and expression of stem cell markers (CD133 and CD44), as well as decreased serum dependence. Conversely, knock-down of HERV-H significantly reduced cancer cell proliferation. Using real-time PCR array, MAPK, Wnt and p53 pathways were all shown to be implicated in HERV-H-mediated oncogenic signalling. Together with previous clinical reports, our data demonstrates HERV-H¿s role in colorectal cancer and highlights the molecular basis underlying its involvement.
URI: http://scholarbank.nus.edu.sg/handle/10635/78638
Appears in Collections:Ph.D Theses (Open)

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