Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.aca.2005.05.060
Title: Advanced analytical tools in proteomics
Authors: Panicker, R.C.
Chattopadhaya, S.
Yao, S.Q. 
Keywords: 2D gel electrophoresis
Bioimaging
Enzyme
Microarray
Peptide
Protein
Protein labeling
Proteomics
Issue Date: 18-Jan-2006
Citation: Panicker, R.C., Chattopadhaya, S., Yao, S.Q. (2006-01-18). Advanced analytical tools in proteomics. Analytica Chimica Acta 556 (1) : 69-79. ScholarBank@NUS Repository. https://doi.org/10.1016/j.aca.2005.05.060
Abstract: Proteomics deals with the study of proteins, their structures, localizations, posttranslational modifications, functions and interactions with other proteins. The mapping of protein structure-function holds the key to a better understanding of cellular functions under both normal and disease states, which is critical for modern drug discovery. However, the study of human proteome presents scientists with a task much more daunting than the human genome project. In fact, the estimated >100,000 different proteins expressed from 30,000 to 40,000 human genes make it extremely challenging, if not impossible with existing protein analysis techniques, to map the entire cellular functions at the translational level. Consequently, there have been rapid advances in the techniques and methods capable of large-scale proteomic studies. Among them, the recently developed high-throughput screening methods have enabled scientists to analyze proteins quickly and efficiently at an organism-wide scale. Herein, we overview some of these emerging tools for high-throughput protein analysis. In particular, we focus on recent advances in the bioassay development, which has provided sensitive and selective tools for high-throughput identification and characterizations of enzymes. Finally, the recently developed bioimaging techniques to visualize and quantify proteins in living cells are also discussed. © 2005 Elsevier B.V. All rights reserved.
Source Title: Analytica Chimica Acta
URI: http://scholarbank.nus.edu.sg/handle/10635/77573
ISSN: 00032670
DOI: 10.1016/j.aca.2005.05.060
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

9
checked on Sep 25, 2018

WEB OF SCIENCETM
Citations

9
checked on Sep 25, 2018

Page view(s)

54
checked on Sep 21, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.