Please use this identifier to cite or link to this item: https://doi.org/10.1002/(SICI)1522-2683(19990701)20:9<1862
Title: Ion-pair solid-phase extraction of biogenic amines before micellar electrokinetic chromatography with laser-induced fluorescence detection of their fluorescein thiocarbamyl derivatives
Authors: Rodríguez, I.
Lee, H.K. 
Li, S.F.Y. 
Keywords: Biogenic amines
Capillary electrophoresis
Fluorescence detection
Micellar electrokinetic chromatography
Solid-phase extraction
Issue Date: 1999
Citation: Rodríguez, I.,Lee, H.K.,Li, S.F.Y. (1999). Ion-pair solid-phase extraction of biogenic amines before micellar electrokinetic chromatography with laser-induced fluorescence detection of their fluorescein thiocarbamyl derivatives. Electrophoresis 20 (9) : 1862-1868. ScholarBank@NUS Repository. https://doi.org/10.1002/(SICI)1522-2683(19990701)20:9<1862
Abstract: Extraction conditions by ion-pair solid-phase extraction of biogenic amines from soy sauce samples were studied. The type of surfactant used as ion-pair reagent, the mode of extraction and the pH were factors studied to enhance the extraction efficiency. High recoveries were obtained using dodecylbenzenesulfonic acid as ion-pair reagent. Octanesulfonate and decanesulfonate did not provide satisfactory recoveries. A dynamic ion-pair solid-phase extraction method was also tested for comparison. The extracted amines were derivatized with fluorescein isothiocyanate, separated by micellar electrokinetic chromatography and detected by laser-induced fluorescence (MEKC-LIF). Because of sample clean-up after extraction and the very high number of theoretical plates obtained with the MEKC-LIF method, identification and quantification of biogenic amines in soy sauce samples could be accomplished. Biogenic amines in soy sauce samples could be separated and quantified with detection limits of the order of 0.1 μg/mL, depending on the sample source.
Source Title: Electrophoresis
URI: http://scholarbank.nus.edu.sg/handle/10635/77457
ISSN: 01730835
DOI: 10.1002/(SICI)1522-2683(19990701)20:9<1862
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