Please use this identifier to cite or link to this item: https://doi.org/10.1016/0021-9673(94)85140-9
Title: Determination of isoelectric points of acidic and basic proteins by capillary electrophoresis
Authors: Yao, Y.J.
Khoo, K.S.
Chung, M.C.M.
Li, S.F.Y. 
Issue Date: 1994
Source: Yao, Y.J., Khoo, K.S., Chung, M.C.M., Li, S.F.Y. (1994). Determination of isoelectric points of acidic and basic proteins by capillary electrophoresis. Journal of Chromatography A 680 (2) : 431-435. ScholarBank@NUS Repository. https://doi.org/10.1016/0021-9673(94)85140-9
Abstract: The isoelectric point (pI) of a protein is of practical importance in many separation procedures, both analytical and preparative. The pI is defined as the pH where the net charge of the protein is zero. Therefore, by plotting the mobilities of the proteins against pH, the intercept at zero mobility should yield the pI value. Isoelectric points have traditionally been determined by isoelectric focusing. In this paper, the potential of capillary electrophoresis as an alternative technique for the determination of pI values of both acidic and basic proteins was investigated. The problem commonly encountered with adsorption of the positively charged proteins with the unprotonated silanol groups of the fused-silica wall is solved by applying a dynamic coating of a polycationic reagent to the wall. The advantages of this technique of determining the pI values are simplicity, speed and minimal sample requirement.
Source Title: Journal of Chromatography A
URI: http://scholarbank.nus.edu.sg/handle/10635/77423
ISSN: 00219673
DOI: 10.1016/0021-9673(94)85140-9
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

39
checked on Mar 21, 2018

WEB OF SCIENCETM
Citations

34
checked on Mar 21, 2018

Page view(s)

28
checked on Apr 20, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.