Please use this identifier to cite or link to this item: https://doi.org/10.1002/bip.20664
Title: Activity based fingerprinting of proteases using FRET peptides
Authors: Sun, H.
Panicker, R.C.
Yao, S.Q. 
Keywords: Activity-based fingerprinting
Catalomics
Enzyme assay
FRET
Peptide
Protease
Issue Date: 2007
Source: Sun, H., Panicker, R.C., Yao, S.Q. (2007). Activity based fingerprinting of proteases using FRET peptides. Biopolymers - Peptide Science Section 88 (2) : 141-149. ScholarBank@NUS Repository. https://doi.org/10.1002/bip.20664
Abstract: We have successfully developed a protease assay using fluorescence resonance energy transfer based peptide libraries, which allows not only general detection of enzymatic activities, but more importantly substrate fingerprinting of proteases from different classes. The method allows the generation of substrate fingerprints of a protease from both the nonprime and prime sites. Therefore, it is well suited for profiling of major metalloproteases such as thermolysin and MMPs. We envisage that this method will provide a useful tool in the emerging field of "Catalomics" for high-throughput studies of proteases. © 2007 Wiley Periodicals, Inc.
Source Title: Biopolymers - Peptide Science Section
URI: http://scholarbank.nus.edu.sg/handle/10635/77393
ISSN: 00063525
DOI: 10.1002/bip.20664
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

22
checked on Feb 21, 2018

WEB OF SCIENCETM
Citations

22
checked on Nov 19, 2017

Page view(s)

36
checked on Feb 24, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.