Systematic optimization of capillary electrophoretic separation of sulphonamides

Abstract

The use of an optimization scheme for the separation of a group of sulphonamides is described. This scheme utilizes an interpretive optimization approach to predict the optimum conditions for the separation of a group of eight sulphonamides. By conducting nine preplanned experiments that span the maximum working range of the system, overall optimum conditions for separation can be obtained. To confirm the validity of the optimization procedure, additional experiments using the optimum conditions predicted by the scheme were performed. The results demonstrated that satisfactory separation could be achieved by using the optimum separation conditions predicted by the scheme. © 1992.