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Title: Single plane illumination fluorescence correlation spectroscopy (SPIM-FCS) probes inhomogeneous three-dimensional environments
Authors: Wohland, T. 
Shi, X. 
Sankaran, J.
Stelzer, E.H.K.
Issue Date: 10-May-2010
Source: Wohland, T., Shi, X., Sankaran, J., Stelzer, E.H.K. (2010-05-10). Single plane illumination fluorescence correlation spectroscopy (SPIM-FCS) probes inhomogeneous three-dimensional environments. Optics Express 18 (10) : 10627-10641. ScholarBank@NUS Repository.
Abstract: The life sciences require new highly sensitive imaging tools, which allow the quantitative measurement of molecular parameters within a physiological three-dimensional (3D) environment. Therefore, we combined single plane illumination microscopy (SPIM) with camera based fluorescence correlation spectroscopy (FCS). SPIM-FCS provides contiguous particle number and diffusion coefficient images with a high spatial resolution in homo- and heterogeneous 3D specimens and live zebrafish embryos. Our SPIM-FCS recorded up to 4096 spectra within 56 seconds at a laser power of 60 DW without damaging the embryo. This new FCS modality provides more measurements per time and more, less phototoxic measurements per sample than confocal based methods. In essence, SPIM-FCS offers new opportunities to observe biomolecular interactions quantitatively and functions in a highly multiplexed manner within a physiologically relevant 3D environment. © 2010 Optical Society of America.
Source Title: Optics Express
ISSN: 10944087
DOI: 10.1364/OE.18.010627
Appears in Collections:Staff Publications

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