Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0378-4347(98)00184-4
Title: Screening of chlorpropamide in horse plasma by high-performance liquid chromatography with ultraviolet absorbance detection, and confirmation by gas chromatography-mass spectrometry
Authors: Chua, H.C.
Stewart, B.
Lim, B.H.
Lee, H.K. 
Keywords: Chlorpropamide
Issue Date: 7-Aug-1998
Citation: Chua, H.C., Stewart, B., Lim, B.H., Lee, H.K. (1998-08-07). Screening of chlorpropamide in horse plasma by high-performance liquid chromatography with ultraviolet absorbance detection, and confirmation by gas chromatography-mass spectrometry. Journal of Chromatography B: Biomedical Applications 712 (1-2) : 243-252. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-4347(98)00184-4
Abstract: A chromatographic method was developed to detect and confirm the presence of chlorpropamide (I) in horse plasma samples, for antidoping control. The plasma sample (1 ml) was extracted with dichloromethane and screened by high-performance liquid chromatography, and confirmation of the drug's presence was accomplished by using gas chromatography-mass spectrometry (GC-MS). The limit of detection was found to be 3.5 ng/ml at a signal-to-noise ratio of three. Derivatization of I with N,O-bis- (trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane allowed for highly stable, accurate and sensitive GC-MS analysis. Plasma samples collected after the administration of diabinese were positive for I (one- five days) in all samples analysed.
Source Title: Journal of Chromatography B: Biomedical Applications
URI: http://scholarbank.nus.edu.sg/handle/10635/76923
ISSN: 15726495
DOI: 10.1016/S0378-4347(98)00184-4
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