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Title: Rapid estimation of octanol-water partition coefficients of pesticides by micellar electrokinetic chromatography
Authors: Wu, Y.S.
Lee, H.K. 
Li, S.F.Y. 
Keywords: Hydrophobicity
Micellar electrokinetic chromatography
Octanol-water partition coefficient
Sodium cholate
Issue Date: Jul-1998
Source: Wu, Y.S.,Lee, H.K.,Li, S.F.Y. (1998-07). Rapid estimation of octanol-water partition coefficients of pesticides by micellar electrokinetic chromatography. Electrophoresis 19 (10) : 1719-1727. ScholarBank@NUS Repository.
Abstract: Micellar electrokinetic chromatography (MEKC) was evaluated as a new technique for the rapid estimation of octanol-water partition coefficient (logK(ow)). Retention measurements for more than 40 reference pesticides with varied structural characteristics and hydrophobicity were carried out in two MEKC systems, based on sodium dodecyl sulfate (SDS) and sodium cholate (SC), respectively. To enable an accurate determination of capacity factors in the SC-MEKC system, cypermethrin (a synthetic pyrethroid insecticide) was utilized instead of Sudan III as the SC micelle tracer, since a few highly hydrophobic pesticides were found to elute after Sudan III. The linear correlation between logarithmic capacity factor (logk') and logK(ow) in the two systems was examined. It was found that, under the typical buffer condition (10 mM sodium phosphate with 60 mM surfactant, pH 7.0), the SDS-MEKC system provided a somewhat wider dynamic range for hydrophobicity (logK(ow) from -1.0 to 4.5). However, the correlation of logk' with logK(ow) was not very high when all the reference pesticides were included in one single calibration set. For the SC-MEKC system, the dynamic range for logK(ow) was in the range of 1.0-5.5, and a good linear correlation existed between logk' and logK(ow) even when all reference pesticides were incorporated into a single calibration group. By comparing the regression line of the reference pesticides with that of a group of simple aromatic derivatives, it was discovered that molecular size and functionality posed a less significant effect on the measurement of logK(ow) in the SC-MEKC system than in the SDS-MEKC system. Thus, SC-MEKC shall be the system of choice for the estimation of logK(ow). The typical error on logK(ow) determination using the current MEKC technique was within 0.5 units, suggesting that MEKC can be a valuable complement to reversed phase high performance liquid chromatography (RP-HPLC) for the indirect determination of logK(ow). Besides maintaining all the advantages of the HPLC approach, the MEKC technique showed some unique benefits, such as better inter-column reproducibility, higher throughput, and less handling of toxic pesticides and solvents.
Source Title: Electrophoresis
ISSN: 01730835
Appears in Collections:Staff Publications

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