Please use this identifier to cite or link to this item: https://doi.org/10.1364/OL.31.001310
Title: Prism-based multicolor fluorescence correlation spectrometer
Authors: Hwang, L.C.
Leutenegger, M.
Gösch, M.
Lasser, T.
Rigler, P.
Meier, W.
Wohland, T. 
Issue Date: 1-May-2006
Citation: Hwang, L.C., Leutenegger, M., Gösch, M., Lasser, T., Rigler, P., Meier, W., Wohland, T. (2006-05-01). Prism-based multicolor fluorescence correlation spectrometer. Optics Letters 31 (9) : 1310-1312. ScholarBank@NUS Repository. https://doi.org/10.1364/OL.31.001310
Abstract: We report the design and application of a prism-based detection system for fluorescence (cross) correlation spectroscopy. The system utilizes a single laser wavelength for the simultaneous excitation of several dyes of different emission spectra. Fluorescence light is spectrally separated with a prismatic setup, and wavelengths are selected by scanning a fiber-coupled avalanche photodiode across the image spots. Multicolor autocorrelations are demonstrated with standard and tandem dyes, and fluorescence cross-correlation measurements of biotinylated nanocontainers and streptavidin are presented. This spectrometer offers high optical stability and no focal volume mismatch for the multicolor detection of molecular dynamics and interactions, with single-molecule sensitivity. © 2006 Optical Society of America.
Source Title: Optics Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/76821
ISSN: 01469592
DOI: 10.1364/OL.31.001310
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