Optimization of separation of porphyrins by micellar electrokinetic chromatography using the overlapping resolution mapping scheme

Abstract

The capillary electrophoretic separation of nine porphyrins having two to eight carboxylic acid side-chains was optimized by using a systematic optimization scheme based on the overlapping resolution mapping method. Three parameters were considered for the optimization, viz., surfactant (sodium dodecyl sulphate) concentration, amount of organic modifier (N,N-dimethyl-formamide) and the ionic strength of the buffer. To utilize the scheme, a set of seven preplanned experiments were performed. Optimum separation conditions were determined which provided satisfactory separation of the porphyrins within an analysis time of ca. 30 min. © 1993.