Please use this identifier to cite or link to this item: https://doi.org/10.1002/elps.200500255
Title: On-line sample enrichment for the determination of proteins by capillary zone electrophoresis with poly(vinyl alcohol)-coated bubble cell capillaries
Authors: Law, W.S. 
Zhao, J.H.
Li, S.F.Y. 
Keywords: Capillary electrophoresis
Field-amplified sample stacking
Poly(vinyl alcohol)-coated bubble cell capillary
Issue Date: Sep-2005
Source: Law, W.S., Zhao, J.H., Li, S.F.Y. (2005-09). On-line sample enrichment for the determination of proteins by capillary zone electrophoresis with poly(vinyl alcohol)-coated bubble cell capillaries. Electrophoresis 26 (18) : 3486-3494. ScholarBank@NUS Repository. https://doi.org/10.1002/elps.200500255
Abstract: Field-amplified sample stacking (FASS) is used to separate basic proteins in a poly(vinyl alcohol)-coated bubble cell capillary. To our knowledge, this is the first paper describing the on-column stacking of proteins (as cations) using FASS in bubble cell capillary. The bubble cell capillary is fabricated using a one-step method. Cetyltrimethylammonium chloride is added into the running buffer to reverse the EOF and, thus, to pump the water plug out during the sample stacking step. The effect of the water plug lengths and sample injection durations were investigated and optimized. The results obtained were compared with those for the normal capillary without bubble cell in terms of resolution and sensitivity enhancement. Under the optimal condition, this method can improve the sensitivity of the peak areas ranging from 5000- to 26 000-fold. The RSDs (n = 5) of the migration time and peak area are satisfactory (less than 0.6 and 12%, respectively). Application of the capillary electrophoresis method with bubble cell, FASS, and UV detection thereby leads to the determination of these proteins at concentrations ranging from 3 to 10 ng/mL, based on a signal-to-noise ratio of 3:1. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Source Title: Electrophoresis
URI: http://scholarbank.nus.edu.sg/handle/10635/76675
ISSN: 01730835
DOI: 10.1002/elps.200500255
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