Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bmcl.2005.02.019
Title: Nanodroplet profiling of enzymatic activities in a microarray
Authors: Uttamchandani, M. 
Huang, X.
Chen, G.Y.J. 
Yao, S.Q. 
Keywords: Coumarin
Enzyme
Fluorescence
Microarray
Protease
Issue Date: 15-Apr-2005
Citation: Uttamchandani, M., Huang, X., Chen, G.Y.J., Yao, S.Q. (2005-04-15). Nanodroplet profiling of enzymatic activities in a microarray. Bioorganic and Medicinal Chemistry Letters 15 (8) : 2135-2139. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bmcl.2005.02.019
Abstract: We describe a generic method for the large-scale functional characterization of enzymes in a microarray. Poly-l-lysine and amine reactive slides were coated with fluorogenic substrates sensitive to proteases and phosphatases. Patterning enzymes on the slides by robotic printing produced spatially addressable, segregated droplets that were simultaneously exposed to the on-chip sensors. Multiple enzymes were profiled using this system that provided fluorescence readouts across temporal and stoichiometric dimensions concurrently on a single microarray substrate. This integrated microarray platform is applicable not only for the functional annotation of proteins, but also for the rapid agonist and antagonist discovery and in performing on-chip kinetics. © 2005 Elsevier Ltd. All rights reserved.
Source Title: Bioorganic and Medicinal Chemistry Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/76614
ISSN: 0960894X
DOI: 10.1016/j.bmcl.2005.02.019
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