Please use this identifier to cite or link to this item: https://doi.org/10.1021/ja200808y
Title: Multicolor, one- and two-photon imaging of enzymatic activities in live cells with fluorescently quenched activity-based probes (qABPs)
Authors: Hu, M.
Li, L. 
Wu, H. 
Su, Y.
Yang, P.-Y.
Uttamchandani, M. 
Xu, Q.-H. 
Yao, S.Q. 
Issue Date: 10-Aug-2011
Source: Hu, M., Li, L., Wu, H., Su, Y., Yang, P.-Y., Uttamchandani, M., Xu, Q.-H., Yao, S.Q. (2011-08-10). Multicolor, one- and two-photon imaging of enzymatic activities in live cells with fluorescently quenched activity-based probes (qABPs). Journal of the American Chemical Society 133 (31) : 12009-12020. ScholarBank@NUS Repository. https://doi.org/10.1021/ja200808y
Abstract: Fluorescence imaging provides an indispensable way to locate and monitor biological targets within complex and dynamic intracellular environments. Of the various imaging agents currently available, small molecule-based probes provide a powerful tool for live cell imaging, primarily due to their desirable properties, including cell permeability (as a result of their smaller sizes), chemical tractability (e.g., different molecular structures/designs can be installed), and amenability to imaging a wide variety of biological events. With a few exceptions, most existing small molecule probes are however not suitable for in vivo bioimaging experiments in which high-resolution studies of enzyme activity and localization are necessary. In this article, we reported a new class of fluorescently Quenched Activity-Based Probes (qABPs) which are highly modular, and can sensitively image (through multiple enzyme turnovers leading to fluorescence signal amplification) different types of enzyme activities in live mammalian cells with good spatial and temporal resolution. We have also incorporated two-photon dyes into our modular probe design, enabling for the first time activity-based, fluorogenic two-photon imaging of enzyme activities. This, hence, expands the repertoire of smart, responsive probes currently available for live cell bioimaging experiments. © 2011 American Chemical Society.
Source Title: Journal of the American Chemical Society
URI: http://scholarbank.nus.edu.sg/handle/10635/76600
ISSN: 00027863
DOI: 10.1021/ja200808y
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

71
checked on Mar 6, 2018

WEB OF SCIENCETM
Citations

70
checked on Mar 6, 2018

Page view(s)

38
checked on Apr 20, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.