Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.chroma.2007.05.067
Title: Large-volume sample stacking with polarity switching for the analysis of bacteria by capillary electrophoresis with laser-induced fluorescence detection
Authors: Yu, L. 
Li, S.F.Y. 
Keywords: Bacteria
Capillary electrophoresis
Large-volume sample stacking
Issue Date: 17-Aug-2007
Source: Yu, L., Li, S.F.Y. (2007-08-17). Large-volume sample stacking with polarity switching for the analysis of bacteria by capillary electrophoresis with laser-induced fluorescence detection. Journal of Chromatography A 1161 (1-2) : 308-313. ScholarBank@NUS Repository. https://doi.org/10.1016/j.chroma.2007.05.067
Abstract: Capillary electrophoresis has been utilized for the rapid analysis of bacteria under specific experimental conditions. In this work, a method of large-volume sample stacking with polarity switching was evaluated for the analysis of bacteria by capillary electrophoresis with laser-induced fluorescence detection in order to enhance the detection sensitivity. The results indicated that the proposed method is not only effective for the focusing of bacterial cells, but also for the separation of mixtures of bacteria. With the optimized conditions, an enhancement factor of around 60-fold was obtained when long sample plug (up to 39.6% of capillary volume) was injected. Moreover, with the help of such stacking method, single, sharp, intense peak with high efficiency was observed without multiple peaks attributable to irregular clusters and aggregates of bacterial cells. This simple stacking approach appears to be promising as a rapid sterility test in various fields of applications. © 2007 Elsevier B.V. All rights reserved.
Source Title: Journal of Chromatography A
URI: http://scholarbank.nus.edu.sg/handle/10635/76437
ISSN: 00219673
DOI: 10.1016/j.chroma.2007.05.067
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