Please use this identifier to cite or link to this item:<211
Title: High-speed chiral separations on microchip electrophoresis devices
Authors: Rodríguez, I.
Jin, L.J.
Li, S.F.Y. 
Keywords: Chiral separation of amino acids
Fluorescein isothiocyanate
Micellar electrokinetic chromatography
Microchip electrophoresis
Issue Date: 2000
Source: Rodríguez, I.,Jin, L.J.,Li, S.F.Y. (2000). High-speed chiral separations on microchip electrophoresis devices. Electrophoresis 21 (1) : 211-219. ScholarBank@NUS Repository.<211
Abstract: High-speed electrophoretic chiral separations have been successfully performed in a microfabricated device by employing cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC). Utilizing short separation channels and relatively high field strengths in combination with small volume-defined injection plugs, and operating in counter-electroosmotic flow conditions, fast and efficient separations of fluorescein insothiocyanate (FITC)-labeled amino acid enantiomers were obtained. Analysis time ranged from 75 s for the most basic amino acids to 160 s for the most acidic ones with associated efficiencies from 7000 up to 28 000 effective plates (100 000 to 395 000 plates/m). Buffer parameters were varied in order to study the effect on chiral resolution. A buffer system consisting of 100 mM borate (pH 9.4), 30 mM of SDS, and 10 mM γ-CD as chiral selector provided adequate resolution of the majority of FITC-amino acid enantiomers tested.
Source Title: Electrophoresis
ISSN: 01730835
DOI: 10.1002/(SICI)1522-2683(20000101)21:1<211
Appears in Collections:Staff Publications

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