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|Title:||High-speed chiral separations on microchip electrophoresis devices|
|Keywords:||Chiral separation of amino acids|
Micellar electrokinetic chromatography
|Source:||Rodríguez, I.,Jin, L.J.,Li, S.F.Y. (2000). High-speed chiral separations on microchip electrophoresis devices. Electrophoresis 21 (1) : 211-219. ScholarBank@NUS Repository. https://doi.org/10.1002/(SICI)1522-2683(20000101)21:1<211|
|Abstract:||High-speed electrophoretic chiral separations have been successfully performed in a microfabricated device by employing cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC). Utilizing short separation channels and relatively high field strengths in combination with small volume-defined injection plugs, and operating in counter-electroosmotic flow conditions, fast and efficient separations of fluorescein insothiocyanate (FITC)-labeled amino acid enantiomers were obtained. Analysis time ranged from 75 s for the most basic amino acids to 160 s for the most acidic ones with associated efficiencies from 7000 up to 28 000 effective plates (100 000 to 395 000 plates/m). Buffer parameters were varied in order to study the effect on chiral resolution. A buffer system consisting of 100 mM borate (pH 9.4), 30 mM of SDS, and 10 mM γ-CD as chiral selector provided adequate resolution of the majority of FITC-amino acid enantiomers tested.|
|Appears in Collections:||Staff Publications|
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