Please use this identifier to cite or link to this item: https://doi.org/10.1002/elan.200804473
Title: Heterogeneous and homogeneous aptamer-based electrochemical sensors for thrombin
Authors: Tan, E.S.Q. 
Wivanius, R.
Toh, C.-S. 
Keywords: Aptamer
Ferrocene
Heterogeneous
Homogeneous
Thrombin
Issue Date: Mar-2009
Citation: Tan, E.S.Q., Wivanius, R., Toh, C.-S. (2009-03). Heterogeneous and homogeneous aptamer-based electrochemical sensors for thrombin. Electroanalysis 21 (6) : 749-754. ScholarBank@NUS Repository. https://doi.org/10.1002/elan.200804473
Abstract: Nanomolar concentrations of thrombin were electrochemically monitored using heterogeneous switch-on and homogeneous switch-off approaches that incorporated ferrocenyl aptamers. For the first time, the heterogeneous approach was coupled to a glucose/glucose oxidase (GOx) amplification-regeneration system which increased its sensitivity by 2 folds with detection limits of 4,3 nM and 2.5 nM in the absence and presence of glucose/GOx, respectively. We also present a new homogeneous system involving the ferrocenyl aptamer binding thrombin in solution causing a significant decrease in its diffusion coefficient. Thus the ferrocene anodic current decreased at an unmodified gold electrode with detection limit of 3.9 nM and 12 times larger linear range than the heterogeneous method. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
Source Title: Electroanalysis
URI: http://scholarbank.nus.edu.sg/handle/10635/76286
ISSN: 10400397
DOI: 10.1002/elan.200804473
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